Wolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics

Table 1 Summary of G. f. fuscipes collection sites, number of individuals used for Wolbachia sequencing, infection prevalence, and sequence diversity

Collection Site	Site Code	Latitude/ Longitude	No. of Host Individuals	Infection Prevalence (%)¹	Average No. of Nucleotide Differences	No. of Haplotypes	H_d
Pallisa	BK	1.02/33.88	2	53.6 (N = 69)	4.5	6	1.000
Bunghazi	BN	0.93/33.98	5	6.7 (N = 15)	4.9	10	0.970
Busime	BU	0.25/33.97	3	29.4 (N = 34)	23.4	5	1.000
Buvuma	BV	0.31/33.30	5	60 (N = 35)	19.1	8	0.927
Junda	JN	1.33/32.74	8	65.1 (N = 43)	11.2	32	0.995
Kakoga	KK	0.37/30.28	3	35.7 (N = 28)	4.8	8	0.972
Masindi	MS	1.63/31.69	6	6.3 (N = 16)	6.0	14	1.000
Murchison Falls	MF	2.28/31.56	3	90 (N=)	7.4	19	0.983
Osuguro	OS	1.53/33.50	2	20 (N = 20)	5.3	3	1.000
Lukaya River	DRC	−4.48/15.31	5	N/A	N/A	N/A	N/A
Gfq Colony²	GF	N/A	5	N/A	N/A	N/A	N/A

¹Infection prevalence data from [26].
²Collection site origin is unknown for the colony maintained at the Slovakia Academy of Science, Bratislava, Slovakia. Tissues were kindly provided by Peter Takac.
³Only the complete dataset was used to calculate diversity statistics in this table because the conservative dataset eliminated individuals.
Measures of Wolbachia groEL nucleotide differences and genetic diversity were calculated using the complete dataset³. H_d refers to groEL haplotype diversity within a site.

ISSN: 2730-7182