Phylogenetic tree of JmjC1. JmjC1 domains retrieved using P. falciparum and T. gondii orthologs as queries, and analyzed phylogenetically using Bayesian inference and maximum-likelihood. Support values for the respective analyses are shown above or to the right of key nodes important to the relative positions of apicomplexan, ciliates and potential sources of HGT. Although alternative sampling strategies, and inclusion of resulting exemplars from various green algae, separated ciliate and apicomplexan sequences in other phylogenetic analyses, in no case was phylogenetic support for a scenario of HGT as strong as support for the ciliate-apicomplexan sister relationship on this tree. Sequences from additional organisms could alter this tentative conclusion, however, given that JmjC1 from Perkinsus, an organism believed to be much closer than ciliates to apicomplexans, branches separately from other alveolates.