Changes in the proportion of prespore cells but not in prestalk cells. (A) Cells expressing lacZ under the cotB promoter or (B) under the ecmA promoter were developed alone or mixed with AX4 or chtB in a 1:1 ratio. Developmental time points (hours) are represented on the x-axis and percentage of stained cells on the y-axis. AX4[cotB/lacZ] mixed with chtB at the 16 and 20 hours time point, shows a significantly lower percentage of stained cells than when mixed with AX4 (for the 16 and 20 hours time points, t-test, p<0.05, n=3). AX4[ecmA/lacZ] mixed with chtB does not show a significant difference in the percentage of stained cells than when mixed with AX4. (For 16, 20 and 24 hour time points, t-test, p>0.26, n=3).