Table 3 Two-way analysis of variance for relative fitness of mutants in five different environments with the environment and the genotype (mutants) treated as fixed effects, and a mixed effect model with the genotype treated as random and the temperature and glucose treated as fixed effects

Source

df

SS

MS

F values

P

Environment

4

1.18349

0.295871

49.0517

<0.0001***

Genotype

2

0.00386

0.001930

0.3200

0.7268

Environment x Genotype

8

0.05311

0.006639

1.1006

0.3679

Residuals

117

0.70572

0.006032

Mixed effects model

Source

df

F values

P

Temperature

1

49.171

<0.0001***

Glucose

1

49.333

<0.0001***

Temperature x Glucose

1

52.560

<0.0001***