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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Evolution and variability of Solanum RanGAP2, a cofactor in the incompatible interaction between the resistance protein GPA2 and the Globodera pallida effector Gp-RBP-1

Figure 3

RanGAP2 variability affects the strength of the Gpa2 -mediated HR in Nicotiana benthamiana leaves. (A) The five RanGAP2 variants and an empty vector (EV) were transiently expressed in N. benthamiana leaves with Gpa2 and either the Gp-Rbp-1 Rook-6 and Rook-4 variants. The strength of the hypersensitive response obtained for each RanGAP2 variant is indicated as follows: (+++) complete collapse and rapid desiccation of the infiltration patch within two days, (++) complete collapse of the infiltration patch by three days post-infiltration, (+) slow and incomplete collapse with residual live cells. (B) Immunoblot with horse radish peroxidase-conjugated anti-FLAG antibody demonstrating relative protein levels of the five transiently expressed RanGAP2 proteins. These five RanGAP2 variants include VRN2-A1 which has a three amino acid deletion (Δ516-518), STN1-A1 (P106 and F237), ADG4-A2 (S106 and L237), ADG8-A3 (P106 and L237) and GRL1-A2 (S106 and F237) which represent the four haplotype combinations at the 106 and 237 positions. An empty vector (EV) was used as a negative control.

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BMC Ecology and Evolution

ISSN: 2730-7182

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