Figure 1

Sampling, morphological data and species tree. (a) Map showing the sampling localities of Rhinolophus affinis used in this study (modified from Mao et al. 2013 [30]). Populations are presented as circles in which individuals are coloured based on the subspecies membership (R. a. himalayanus: blue; R. a. macrurus: orange; R. a. hainanus: green); (b) Plot showing echolocation call frequency (kHz) and forearm (mm) data for a subset of bats from the three subspecies (R. a. himalayanus, n = 43; R. a. macrurus, n = 39; R. a. hainanus, n = 32). Downward and upward triangles correspond to male and female bats, respectively. Full details are provided in Additional file 3: Table S3; (c) Species tree based on all nuclear markers using BEST.