Figure 1
Genetic variation in E. madsenii , E. rhopalostele , E. falcisepalum and their hybrids across three hybrid zones. (A) Statistical parsimony network of plastid haplotypes based on sequences from six chloroplast DNA regions (trnL-trnF, rps16, rpoC1, psbK-psbI, matk and rbcl). A circle’s size is proportional to the haplotype frequency. Small empty circles represent single mutational steps. (B) Posterior probabilities (q) for the three hybrid zones of Epidendrum madsenii ‘MAD’ and E. falcisepalum ‘FAL’ (HZ1) and E. madsenii ‘MAD’ E. rhopalostele ‘RHO’ (HZ2 and HZ3) analyzed with STRUCTURE and NEWHYBRIDS. Individuals identified in the field, based on morphological characters, are delimited by dashed lines. Each vertical bar represents an individual. The proportion of color in each bar represents an individual’s assignment probability, according to different categories (pure parental species, F1 and F2 hybrids, and the respective backcrosses).