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Table 1 Hierarchical analysis of molecular variance (AMOVA) of ITS and Tpi sequences and nSSRs from 14 populations of Platycrater arguta var. sinensis (East China) and var. arguta (South Japan)

From: A strong ‘filter’ effect of the East China Sea land bridge for East Asia’s temperate plant species: inferences from molecular phylogeography and ecological niche modelling of Platycrater arguta(Hydrangeaceae)

Data sets/regional grouping of populations Source of variation d.f. Sum of squares Variance components Variance explained (%) F- or R-statistics (P)
ITS       
var. sinensis (China) vs. var. arguta (Japan)
  Among groups 1 528.415 13.954 70.28 FCT = 0.703**
  Among populations within groups 12 312.118 4.912 24.74 FSC = 0.833**
  Within populations 58 57.315 0.988 4.98 FST = 0.950**
Tpi       
var. sinensis (China) vs. var. arguta (Japan)
  Among groups 1 357.190 4.053 66.70 FCT = 0.667**
  Among populations within groups 12 175.449 0.979 16.11 FSC = 0.484**
  Within populations 187 195.305 1.044 17.19 FST = 0.828**
nSSRs       
var. sinensis (China) vs. var. arguta (Japan)
  Among groups 1 25515.13 96.12 13.52 RCT = 0.135**
  Among populations within groups 12 7106.99 178.93 25.17 RSC = 0.290**
  Within populations 530 231038.92 435.92 61.31 RST = 0.390**
  1. d.f., degrees of freedom; grouping of populations: Chinese group (populations C1–C7), Japanese group (populations J1–J7; for population codes see Additional file 1: Table S1, Supporting information); ** P < 0.001.
  2. Estimators for Tpi and ITS were calculated based on the infinite alleles model (F-statistics) and those for nSSRs on the stepwise mutation model (R-statistics).