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Figure 3 | BMC Evolutionary Biology

Figure 3

From: A comparative study of genome organization and inferences for the systematics of two large bushcricket genera of the tribe Barbitistini (Orthoptera: Tettigoniidae: Phaneropterinae)

Figure 3

C-, DAPI (blue), and CMA 3 (green) stained heterochromatin and selected chromosomes (with C-bands, DAPI, CMA 3 , 18S rDNA, NOR) of spermatogonial metaphase in I. major (a) and I. modestior from the Bulgarian population (b) as well as of diplotene in P. martinae (c) and Isophya sp. (d). In the selected chromosomes, C/DAPI/CMA3 blocks were located very close to each other, but bright CMA3 signals coincided with 18S rDNA and active NORs (a,b); thick C-bands were DAPI-, whereas bright CMA3+ signals were co-localized with rDNA-FISH/NOR signals (c,d); heteromorphism in the paracentromeric region of 1/2 in Isophya sp. (d) seems to be corroborated by all staining methods. The arrows (left panels) indicate selected chromosomes (right panels). Bar = 10 μm.

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