Figure 4

Phenotype changes in RCAS moa tbx5 chick embryos. Hamburger Hamilton (HH) stage 14 chick embryos were injected in the presumptive hindlimb region with RCAS moa tbx5 and subjected to electroporation. Embryos were harvested at HH stage 40 and stained with Victoria blue. Control HH stage 40 wing and leg are shown. Digits from Victoria blue stained control limbs are numbered. Feather growth in RCAS moa tbx5 electroporated chick embryo hindlimbs is shown by the orange arrow heads. Victoria blue stained RCAS moa tbx5 hindlimbs show a wing-like digit pattern with talon reduction (red arrow heads) and conversion of hindlimb digit 4 to wing-type digit 3 (black arrow head).