Figure 1

Alignment of DJ- 1 proteins from representative fungal species. Candidate fungal DJ-1 proteins are from six ascomycetes, nine basidiomycetes and six basal fungal species. Accession numbers of fungal DJ-1 proteins are used; see Table 1 for details. DJ-1 proteins from human (HsDJ-1), D. melanogaster (DmDJ-1α), C. elegans (cDJR-1.1), Bombyx mori (BmDJ-1) and A. thaliana (AtDJ-1A), EcYajL, EcHsp31 and ScHsp31 are included for comparison. For AtDJ-1A, only the C-terminal region is used for comparison. The alignment was constructed using Clustal W [91] and adjusted manually to improve the alignment. Identical residues are on a black ground and conserved residues shared in gray. The conserved catalytic triad residues of DJ-1 proteins and Hsp31 proteins are indicated below the alignment by stars and filled triangles, respectively. Hyphens represent gaps introduced into sequences for maximum alignment.