Figure 4
From: Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe
HPLC analysis of products formed by Hsp3101 (A), Hsp3102 (B) and ScHsp31, (C) SpDJ-1 (D). Purified recombinant Hsp3101 (257 μg), Hsp3102 (240 μg), ScHsp31 (114 μg) or SpDJ-1 (450 μg) was mixed with 10 mM MG and incubated for 30 min. Aliquots of the reaction mixtures were analyzed using a Ultimate AQ-C18 column (4.6 × 150 mm) eluted at a flow rate of 1.0 ml/min with 10 mM H3PO4 (pH 2.5). The lactate peak marked by an inverted triangle was identified by comparison with the retention time of commercial lactate.