Figure 3

Phylogenetic tree of the Runx protein family. Maximum likelihood tree calculated with the assumption of a molecular clock from the multiple amino acid sequence alignment shown in Figure 2. The numbers at each node are the bootstrap support values obtained by maximum likelihood (no molecular clock assumed), neighbour joining, and maximum parsimony, in that order. The dashes (-) by the branch leading to the Ciona gene (CiRunt) indicate that the maximum likelihood and maximum parsimony methods did not support that node (these methods each place CiRunt on a branch with TrRunx1, with support values of 50 and 52, respectively). Branches with unresolved topology were collapsed. Coloured branches are used to highlight the vertebrate lineage (green) and the insect lineage (red). The shading highlights the deuterostome (blue) and protostome (pink) representatives in the sample. Abbreviations for species names are the same as in Figures 1 and 2.