Figure 2

CLUSTALW [26] was used for the multiple sequence alignment of the full length Xanthomonas axonopodis PNP-like protein and its homologs obtained from BLASTp searches against the NCBI database. An alignment of 287 amino acids with a score of 17253 was obtained and after discarding all columns with gaps the alignment length was reduced to 89 amino acids with a score of 9705. The Neighbor-Joining tree of the 89 amino acid alignment was constructed using MEGA2 [33]. Bootstrap values are shown on the branches and the root was placed at the mid-point of the tree. Sequences are named by abbreviations of the species name followed by the NCBI accession number. Abbreviations: At – Arabidopsis thaliana, Ca – Cicer arietium, Cj – Citrus jambhri, Es – Erucastrum strigosum, Gh – Gossypium hirsutum, Hh – Hedera helix, Os – Oryza sativa, Xa – Xanthomonas axonopodis pv. Citri str. 306 (in red).