Figure 1

Multiple sequence alignment of the relaxin-like peptide family. Amino acid sequences of the B and A domains only were aligned using ClustalW, then edited by hand using Seaview to remove gaps. This alignment was then used for all phylogenetic analyses. Newly identified sequences are highlighted in bold and italics. Invariant cysteine residues are indicated by asterisks (*) and the relaxin specific B-chain motif [RxxxRxxI/V] is shown. Sequences are clustered into subfamilies (A and B) based on primary sequence similarity and phylogenetic analysis. Hsa = Homo sapiens, Pt = Pan troglodytes, Mmul = Maca mulatta, Mm = Mus musculus, Rn = Rattus norvegicus, Cf = Canis familiaris, Ss= Sus scrofa, Re = Rana esculenta, Me = Macropus eugenii, Xl = Xenopus laevis, Xt = Xenopus tropicalis, Dr = Danio rerio, Tr = Takifugu rubripes, Gg = Gallus gallus, Om = Oncorhynchus mykiss.