Figure 4

Alignment of human and mouse β-defensin peptides showing sites of selection. Sites of significant positive and negative selection (according to SWAPSC, PAML and ADAPTSITE) in primate and mouse lineages are indicated by asterisks. Hashes indicate every tenth position in the alignment. Hominidae, Cercopithecidae, Hylobatidae and Callitrichidae are abbreviated to Hom, Cer, Hyl and Cal respectively. The positions of the beta strands and alpha helix in DEFB1 are indicated, the residues within the prepropeptide region are those before the alpha helix. The horizontal lines under the alignment denote the results of different analyses: SWAPSC results for primate and mouse lineages individually, SWAPSC sites under positive and negative selection in different branches within primates (Primate +/-) and mouse (Mouse +/-), PAML results for sites under positive selection, ADAPTSITE results for sites under positive an negative selection, sites implicated as under positive selection by two or more different programs for primate (Primate ++ and Primate --) and mouse (Mouse ++ and Mouse--) lineages.