Figure 1
From: Genome wide survey of G protein-coupled receptors in Tetraodon nigroviridis
Comprehensive approach for the identification and validation of Tnig-GPCRS. All GPCR sequences from GPCRDB were compared against Tetraodon proteome database using BLASTP and hits were searched against GPCRDB using reverse BLAST. As complementary approaches, Tetraodon sequences were compared using Hmmpfam against Pfam and RPS-BLAST against CDD respectively. Finally, GPCR sequences are subjected to phylogenetic analysis as described in Methods.