Figure 9

eIF4E-family members from some species of Protista show extension or compaction. A. An alignment of amino acid sequences representing the conserved core regions of eIF4E-family members from Alveolata, Stramenopiles, the Haptophyceae E. huxleyi and of H. sapiens eIF4E-1, and M. musculus eIF4E-2A and eIF4E-3. Green boxes indicate amino acids extensions relative to Class I, II, or III eIF4E-family members from other species. B. An alignment of the complete predicted amino acid sequences of predicted eIF4E-family members from C. merolae, G. theta nucleomorph, and E. cuniculi, and from H. sapiens eIF4E-1, and M. musculus eIF4E-2A and eIF4E-3. Residues shaded in light blue indicate regions N- and C- terminal to the conserved core of the respective eIF4E-family member. Residues shaded in greenindicate variations at positions equivalent to Val-69 and Trp-73 of H. sapiens eIF4E-1. For both A and B: amino acid residues within the alignment are highlighted as described in the legend to Figure 3A with the exception that residues shaded in grey indicate amino acids similar in greater than 80% (A) or 70% (B) of the sequences shown. Numbers to the right of the alignments represent distances of amino acids with respect to the N-terminal Met residue (black) or, for eIF4E-family members for which the N-terminal Met could not be predicted, from the first residue shown (red). eIF4E-family members for which names are shown in red indicate that only a portion of the core region for that member could be estimated. eIF4E-family members for which names are shown in blue indicate that sequences were predicted using genomic sequence data.