Figure 5

A. Analyses of long-branch indicators of Glaucocystis, and the most slowly evolving sequences of the animal, fungal, plant and amoebozoan clades based on analyses shown in figure 3. Lightly shaded bars show attachment of random sequences and darker bars unique substitutions at highly conserved sites. Thalassiosira, Glaucosphaera (the red alga with the least "long-branch" tendencies) and Stylonychia were included in the tabulation of unique substitutions (but not with random sequence analyses), to provide additional evidence that the sites in question were under strong stabilizing selection across eukaryotic diversity. With this sub-alignment, only single unique substitutions were scored. B. Five taxon ML tree (JTT + Γ + I) with branch lengths showing sequence change across branches and internodes. Unit is expected changes per amino acid position.