Figure 3

Adherence and invasion of S. typhimurium strains containing a deletion of island 4305 and of E. coli and P. putida strains containing the cloned island 4305 region. The indicated bacterial strains were assayed for their adherence and invasion of the HT-29 colonic epithelial cell line as described in the Materials and Methods. The percent adherence is given as the percentage of the inoculum that remained tissue culture cell-associated after washing with buffer. The percent invasion is given as the percentage of adhered bacterial cells that survived gentamycin treatment. The invA strain contains a well-characterized mutation that results in severely reduced invasion of S. typhimurium into cultured epithelial cells and serves as a control comparison.