Figure 5

RT-PCR analysis of cloned island 4305 gene expression in different Gram negative hosts. Representative examples of RT-PCR analysis of cloned island 4305 and adjacent gene expression in a range of Gram negative hosts is presented. A full catalog of the results is listed in Table 3. Lanes in the agarose gels are as described in Figure 4. The boxed pictures indicate where expression of the tested gene is not detectable. All Gram negative hosts containing the plasmid R995 gave the expected negative results in the RT-PCR analysis (data not shown).