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Table 2 Differences observed by comparison of grape chloroplast genome sequences with EST sequences obtained by BLAST search in Genbank.

From: Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids

Gene Gene size (bp) EST Sequencea Number of variable sites Variation typeb Position(s)c Amino acid change
atpI 744 1–744 2 G-A
C-U
453
629
A-A
L-S
clpP 591 62–366 5 G-A
A-U
64
65
D-I
     T-A
A-U
70
71
Y-I
     C-U 364 R-W
matK 1509 416–1262 2 C-U
G-A
448
1260
H-Y
K-K
ndhA 1092 1–553 1 T-C 553 L-L
ndhI 504 77–356 1 C-U 162 R-R
PetB 648 4–648 1 G-A 5 S-N
PetD 483 6–483 1 G-A 7 V-I
PsbA 1062 397–1014 2 T-C
G-A
420
463
R-R
A-T
rpl22 462 1–462 1 C-U 46 Q-Stop
  1. aSequence analyzed coordinates based on the gene sequence, considering the first base of the initiation codon as bp 1. bVariation type: (nucleotide in genomic DNA) – (nucleotide in mRNA). cVariable position is given in reference to the first base of the initiation codon of the gene sequence.