Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids

Table 2 Differences observed by comparison of grape chloroplast genome sequences with EST sequences obtained by BLAST search in Genbank.

Gene	Gene size (bp)	EST Sequence^a	Number of variable sites	Variation type^b	Position(s)^c	Amino acid change
atpI	744	1–744	2	G-A C-U	453 629	A-A L-S
clpP	591	62–366	5	G-A A-U	64 65	D-I
				T-A A-U	70 71	Y-I
				C-U	364	R-W
matK	1509	416–1262	2	C-U G-A	448 1260	H-Y K-K
ndhA	1092	1–553	1	T-C	553	L-L
ndhI	504	77–356	1	C-U	162	R-R
PetB	648	4–648	1	G-A	5	S-N
PetD	483	6–483	1	G-A	7	V-I
PsbA	1062	397–1014	2	T-C G-A	420 463	R-R A-T
rpl22	462	1–462	1	C-U	46	Q-Stop

^aSequence analyzed coordinates based on the gene sequence, considering the first base of the initiation codon as bp 1. ^bVariation type: (nucleotide in genomic DNA) – (nucleotide in mRNA). ^cVariable position is given in reference to the first base of the initiation codon of the gene sequence.

ISSN: 2730-7182