Table 5 Primer sequences used to amplify PRP8 intein encoding sequences from euascomycetes.
From: The distribution and evolutionary history of the PRP8 intein
Primer name | Relevant species | Primer sequence |
|---|---|---|
Aspfum-F1 | A. fumigatus, | 5' acagatgtcatccaagc 3' |
Aspfum-F2 | (including | 5' tgggaaagagcatgccttgc 3' |
Aspfum-F4 | Afu. Var. ellipticus | 5' gaaccaggaaatggagacg 3' |
Aspfum-Rv1 | and | 5' ttcaacggtatcgtagcg 3' |
Aspfum-Rv4 | Neosartorya fischeri) | 5' acagagtgaaccgacg 3' |
Liu_1 | Fumigati spp. and | 5' atgaagagcaa [tc]cc [agct]tt [tc]tggtggac 3' |
Liu_2 | A giganteus | 5' gcattcgtgag [tc]tt [ct]tt [ag]aa [tc]ttcat 3' |
Ani-Fn | A. nidulans | 5' agcttgtcttgccaac 3' |
Ani-Fs | A. nidulans | 5' acaaagacagaccaaccaataaacggattg 3' |
Ani-Ra | A. nidulans | 5' actgttatgcagtacaacatag 3' |
Ani-Rsn | A. nidulans | 5' tctcccaggagtctcgacgctaga 3' |
Pb_Fwd1 | P. brasiliensis | 5' catatctctggaactgtggcag 3' |
Pb_Fwd2 | P. brasiliensis | 5' gcagttgggtattgacacagtgc 3' |
Pb_Rev1 | P. brasiliensis | 5' gcactgtgtcaatacccaactgc 3' |
Pb_Rev2 | P. brasiliensis | 5' gcgttcgtcaacttcttgaacttcat 3' |
Pb_Rev3 | P. brasiliensis | 5' gctttgtgctgcctcgttaacg 3' |
Para_b-F3 | P. brasiliensis | 5' gatagaagtcgcacg 3' |
Para_b-Rv4 | P. brasiliensis | 5' gggtcggtttatgc 3' |