Figure 2
From: Genetic structure and evolution of the Vps25 family, a yeast ESCRT-II component
Multiple tissue PCR analysis of the expression of human VPS25. A panel of normalised, first strand cDNAs, ready for quantitative PCR, was screened using primers designed to specifically amplify 547 bp portion of VPS25 mRNA. Expression of VPS25 after 30 (upper panel) or 38 cycles (lower panel) was examined. After 38 cycles saturation of most reactions had occurred, but is provided to clearly demonstrate expression in brain. A PCR product of approximately 550 bp was detected in all human tissues examined. The arrow indicates 500 bp marker. N = negative control (no DNA). P = positive control (human VPS25 cDNA).