Intragenomic comparisons of the genome signature of 20 bacterial species (see Additional File 4). Highlighted are 10 (Burkholderia sp. 383 chromosome 1 vs. 3), 13 (Burkholderia xenovorans LB400 chromosome 1 vs. 3), 21 (Leptospira interrogans serovar Copenhageni str. Fiocruz L1–130 chromosome I vs. II) and 22 (Leptospira interrogans serovar Lai str. 56601 chromosome I vs. II). The average genomic dissimilarity between chromosomes of the same species is 0.016 (red line).