Table 5 Role of the positively selected sites in the discrepancy between feruloyl esterase A and lipase activities.
Three dimensional localization | Positively selected sites | Site directed mutagenesis | & Role on Lip/FAE discrepancy | References |
|---|---|---|---|---|
1. Flap region (69–80) | 71,75,78,80 | 80 | catalysis and substrate discrimination | [45,46] |
2. Catalytic vicinity | 100 | 100 | substrate recognition | [47] |
3. Loop (226–244) | 236,238,244 | - | structured plasticity to the substrate binding site | [23,48] |