Phylogenetic relationship of COI lineages. (A) Cladogram representing lineage classification of Cx. annulirostris and Cx. palpalis. Lineage names are indicated on the right of the tree. The tree was rooted using Cx. sitiens and the outgroup is not shown. Maximum Likelihood bootstrap values for individual alphabetically labelled branches are summarized in Table B. (B) Three different dataset selections of 18 haplotypes from the original alignment of 182 haplotypes were used to calculate the bootstrap support; for details see Materials and Methods. Values were calculated using Maximum Likelihood in PhyML 2.4.4 (500 replicates) and PAUP* 4b10 (100 replicates); for details see Materials and Methods. Branches not supported by the analysis and dataset are indicated as * in the table and indicate paraphyly of the selected Cx. annulirostris ann-AUS sequences; n.a. indicates value not available.