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Table 1 K d and K i values (mean ± SEM) obtained from saturation and competition curves, respectively, for human and lamprey melanocortin peptide analogues binding lamprey MCa receptor and human MC1, MC3, MC4 and MC5 receptors. * P < 0.01

From: Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypes

 

lamprey MCa

human MC1a

human MC3e

human MC4e

human MC5b

Ligand

(nmol·L-1)

(nmol·L-1)

(nmol·L-1)

(nmol·L-1)

(nmol·L-1)

[125I] NDP-MSH (K d )

2.46 ± 0.68

0.109 ± 0.062

0.412 ± 0.121

1.78 ± 0.36

5.05 ± 1.00

NDP-MSH (K i )

17.8 ± 9.9

0.046 ± 0.011

0.319 ± 0.064

1.96 ± 0.39

2.39 ± 0.10

α-MSH (K i )

248 ± 59*

0.210 ± 0.089

21.2 ± 5.3

522 ± 122

8240 ± 1670

β-MSH (K i )

54.7 ± 5.1

2.53 ± 0.93

15.1 ± 3.4

387 ± 208

14400 ± 1670

γ1-MSH (K i )

684 ± 7*

2.68 ± 0.35b

7.45 ± 2.55

51800 ± 12000

42600 ± 6600

ACTH (1–17) (K i )

51.1 ± 3.2

0.230 ± 0.061c

14.0 ± 4.5c

419 ± 62c

4240 ± 1200c

ACTH (1–24) (K i )

56.4 ± 10.8

0.209 ± 0.052c

32.8 ± 6.7

755 ± 151

2760 ± 780c

ACTH (1–39) (K i )

291 ± 20*

3.95 ± 0.67c

135 ± 22c

2170 ± 120c

4920 ± 610c

MTII (K i )

71.0 ± 3.3

0.686 ± 0.109d

52.6 ± 7.9

6.60 ± 0.82f

46.1 ± 7.9d

HS024 (K i )

72.5 ± 1.4

18.6 ± 3.3d

15.1 ± 3.0

0.341 ± 0.089

3.29 ± 1.15d

MSH-A (K i )

1505 ± 262*

    

MSH-B (K i )

352 ± 34

    

ACTH(1–31) (K i )

497 ± 33

    
  1. Data taken from a [38]; b [39]; c [40]; d [41]; e [42]; f [43]