From: Analysis of septins across kingdoms reveals orthology and new motifs
a. Established Motifs and Extensions | ||||
---|---|---|---|---|
Amino acid | Frequency (162 total) | Bitscore | Other Residues+ | Â |
G1 motif (SceCdc3 126–135) | ||||
G | 162(100%) | 4.3 | / | Â |
x | / | / | / | Â |
x | / | / | / | Â |
G | 152(94%) | 3.6 | N(4) | Â |
x | / | / | / | Â |
G | 160(99%) | 4.2 | / | Â |
[KR] | 158(98%) | 3.4/0.4 | / | Â |
[ST] | 154(95%) | 2/1.1 | / | Â |
[TS]* | 157(97%) | 3.4/0.3 | A(3) | Â |
[LF]* | 156(96%) | 1.9/1.2 | M(3) | Â |
G3 motif (SceCdc3 204–209) | ||||
D | 135(83%) | 2.7 | E(6), N(4), S(4), L(3), T(3) | Â |
T* | 141(87%) | 3.1 | A(7), S(5) | Â |
[PV]* | 139(86%) | 2.1/0.3 | E(6), H(3) | Â |
G | 153(94%) | 3.8 | N(4) | Â |
x* | / | / | / | Â |
G* | 150(93%) | 3.7 | E(6) | Â |
G4 motif (SceCdc3 280–289) | ||||
N* | 156(96%) | 3.8 | T(5) | Â |
x* | / | / | / | Â |
x* | / | / | / | Â |
P* | 159(98%) | 4 | L(2) | Â |
x* | / | / | / | Â |
I* | 147(91%) | 3.3 | L(9), V(5) | Â |
x | / | / | / | Â |
K | 148(91%) | 3.4 | R(11) | Â |
x | / | / | / | Â |
D | 160(99%) | 4 | / | Â |
* Modifications of previously defined motifs. | ||||
+ Most common examples of other residues; not all possibilities shown. | ||||
b. New Septin Motifs | ||||
Amino acid | Frequency | Bitscore | Other Residues+ | Â |
Sep1 motif (SceCdc3 237–242) | ||||
E | 156(96%) | 3.9 | D(5) | Â |
x | / | / | / | Â |
x | / | / | / | Â |
x | / | / | / | Â |
x | / | / | / | Â |
R | 158(98%) | 3.9 | T(2) | Â |
Sep2 motif (SceCdc3 247–259) | ||||
D | 156(96%) | 3.9 | E(2), G(2) | Â |
x | / | / | / | Â |
R | 150(93%) | 3.5 | H(5) | Â |
[VI] | 155(96%) | 1.9/1 | P(4) | Â |
H | 153(94%) | 3.6 | D(4), Q(4) | Â |
x | / | / | / | Â |
x | / | / | / | Â |
x | / | / | / | Â |
[YF] | 156(96%) | 3.2/0.3 | L(5) | Â |
F | 147(91%) | 3.3 | L(9) | Â |
[IL] | 153(94%) | 2.9/0.3 | V(8) | Â |
x | / | / | / | Â |
P | 142(88%) | 3.1 | A(10), S(3) | Â |
Sep3 motif (SceCdc3 261–268) | ||||
G | 140(86%) | 3 | S(6) | Â |
x | / | / | / | Â |
x | / | / | / | Â |
L | 151(93%) | 3.2 | D(5), I(4), V(3) | Â |
x | / | / | / | Â |
x | / | / | / | Â |
x | / | / | / | Â |
D | 156(96%) | 3.9 | E(6) | Â |
Sep4 motif (SceCdc3 364–365) | ||||
W | 149(92%) | 3.7 | D(4) | Â |
G | 149(92%) | 3.6 | / | Â |
+ Most common examples of other residues; not all possibilities shown. | ||||
c. Single Conserved Position | ||||
SceCdc3 | Â | Â | Â | Â |
Position | Amino Acid | Frequency | Bitscore | Other Residues+ |
117 | G | 140(86%) | 3.3 | P(4) |
295 | [ED] | 152(94%) | 3.1/0.2 | H(5) |
300 | K | 150(93%) | 3.6 | R(9) |
339 | P | 150(93%) | 3.5 | D(6) |
360 | R | 150(93%) | 3.4 | / |
396 | T | 150(93%) | 3.4 | S(5) |
+ Most common examples of other residues; not all possibilities shown. |