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Table 2 Conserved motifs and single residues in septins.

From: Analysis of septins across kingdoms reveals orthology and new motifs

a. Established Motifs and Extensions

Amino acid

Frequency (162 total)

Bitscore

Other Residues+

 

G1 motif (SceCdc3 126–135)

G

162(100%)

4.3

/

 

x

/

/

/

 

x

/

/

/

 

G

152(94%)

3.6

N(4)

 

x

/

/

/

 

G

160(99%)

4.2

/

 

[KR]

158(98%)

3.4/0.4

/

 

[ST]

154(95%)

2/1.1

/

 

[TS]*

157(97%)

3.4/0.3

A(3)

 

[LF]*

156(96%)

1.9/1.2

M(3)

 

G3 motif (SceCdc3 204–209)

D

135(83%)

2.7

E(6), N(4), S(4), L(3), T(3)

 

T*

141(87%)

3.1

A(7), S(5)

 

[PV]*

139(86%)

2.1/0.3

E(6), H(3)

 

G

153(94%)

3.8

N(4)

 

x*

/

/

/

 

G*

150(93%)

3.7

E(6)

 

G4 motif (SceCdc3 280–289)

N*

156(96%)

3.8

T(5)

 

x*

/

/

/

 

x*

/

/

/

 

P*

159(98%)

4

L(2)

 

x*

/

/

/

 

I*

147(91%)

3.3

L(9), V(5)

 

x

/

/

/

 

K

148(91%)

3.4

R(11)

 

x

/

/

/

 

D

160(99%)

4

/

 

* Modifications of previously defined motifs.

+ Most common examples of other residues; not all possibilities shown.

b. New Septin Motifs

Amino acid

Frequency

Bitscore

Other Residues+

 

Sep1 motif (SceCdc3 237–242)

E

156(96%)

3.9

D(5)

 

x

/

/

/

 

x

/

/

/

 

x

/

/

/

 

x

/

/

/

 

R

158(98%)

3.9

T(2)

 

Sep2 motif (SceCdc3 247–259)

D

156(96%)

3.9

E(2), G(2)

 

x

/

/

/

 

R

150(93%)

3.5

H(5)

 

[VI]

155(96%)

1.9/1

P(4)

 

H

153(94%)

3.6

D(4), Q(4)

 

x

/

/

/

 

x

/

/

/

 

x

/

/

/

 

[YF]

156(96%)

3.2/0.3

L(5)

 

F

147(91%)

3.3

L(9)

 

[IL]

153(94%)

2.9/0.3

V(8)

 

x

/

/

/

 

P

142(88%)

3.1

A(10), S(3)

 

Sep3 motif (SceCdc3 261–268)

G

140(86%)

3

S(6)

 

x

/

/

/

 

x

/

/

/

 

L

151(93%)

3.2

D(5), I(4), V(3)

 

x

/

/

/

 

x

/

/

/

 

x

/

/

/

 

D

156(96%)

3.9

E(6)

 

Sep4 motif (SceCdc3 364–365)

W

149(92%)

3.7

D(4)

 

G

149(92%)

3.6

/

 

+ Most common examples of other residues; not all possibilities shown.

c. Single Conserved Position

SceCdc3

    

Position

Amino Acid

Frequency

Bitscore

Other Residues+

117

G

140(86%)

3.3

P(4)

295

[ED]

152(94%)

3.1/0.2

H(5)

300

K

150(93%)

3.6

R(9)

339

P

150(93%)

3.5

D(6)

360

R

150(93%)

3.4

/

396

T

150(93%)

3.4

S(5)

+ Most common examples of other residues; not all possibilities shown.