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Table 1 Primers used for real-time PCR gene expression analysis. F and R refer to forward and reverse primers, respectively.

From: Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): phylogeny and tissue- and development-specific expression

Target

Primers

Ā Ā 

Fragment size (bp)

Ā 

Primer pair name

Sequence

5'-position

Ā 

RPS2

SserpS2ā€¢1

5'-CCAAGCTGTCGATTGTCCCGGTCA-3' (F)

434

127

Ā 

SserpS2ā€¢2

5'-CGGGGGGCAGGGATGAGACG-3' (R)

560

Ā 

RPS3a

SserpS3aā€¢1

5'-TCAGAAAGACCTCCTACGCCCAGCA-3' (F)

450

94

Ā 

SserpS3aā€¢2

5'-AGATCATTGGTCTGAACCTCACGGGTCA-3' (R)

543

Ā 

RPS15

SserpS15ā€¢1

5'-CATGGTTGGCGTGTACAATGGCAAA-3' (F)

300

116

Ā 

SserpS15ā€¢2

5'-GGCGACCGTGCTTGACTGGCTTG-3'(R)

415

Ā 

RPS27-1

SserpS27-1ā€¢1

5'-CCCGAGGAGGAGAAGAGGAGGCACA-3' (F)

64

124

Ā 

SserpS27-1ā€¢2

5'-CTGTCTGAGCGTGACTGAACACCGTCGT-3' (R)

187

Ā 

RPS27-2

SserpS27-2ā€¢1

5'-GCTAAAGACCTCCTCCACCCTGCCATT-3' (F)

72

130

Ā 

SserpS27-2ā€¢2

5'-ACACAGTTGTGATTTTGTAGCAGCCTGGAC-3' (R)

201

Ā 

RPS27a

SserpS27aā€¢1

5'-GCGTGAGTGTCCGGCTGACGA-3' (F)

431

87

Ā 

SserpS27aā€¢2

5'-GTGAGGCAGCACTTCCCGCAGT-3' (R)

517

Ā 

RPS28

SserpS28ā€¢1

5'-CGATAGTTCCCGCTGAAGCTGTGAGGTG-3' (F)

206

95

Ā 

SserpS28ā€¢2

5'-GAGAATGTGAGGGATGTCCGCCGTTG-3' (R)

300

Ā 

RPS29

SserpS29ā€¢1

5'-AGGCAGTACGCTAAAGACATCGGCTTCGTG-3' (F)

135

121

Ā 

SserpS29ā€¢2

5'-GTGCTGAATTATCCCATCATCTTGGCTGGT-3' (R)

255

Ā 

GAPDH

SseGAPDH231ā€¢1

5'-AGCCACCGTGTCGCCGACCT-3' (F)

1001

107

Ā 

SseGAPDH231ā€¢2

5'-AAAAGAGGAGATGGTGGGGGGTGGT-3' (R)

1107

Ā 

Ubiquitin

SseUBā€¢1

5'-AGCTGGCCCAGAAATATAACTGCGACA-3' (F)

289

93

Ā 

SseUBā€¢2

5'-ACTTCTTCTTGCGGCAGTTGACAGCAC-3' (R)

381

Ā