Figure 6

Identification of amino acid residues conserved in all Nox/Duox proteins. The single letter amino acid code is used, and consensus amino acid residues and locations are identified based on the alignment in Additional file 5. In the column labeled "consensus", "a" refers to hydrophobic side-chain amino acids. In the column labeled "location", "TM" refers to TM α-helix; and "TM3-heme" and "TM5-heme" refer to predicted heme-ligating histidine residues. NADPH1-2, NADPH2-3, and NADPH3-4 refer to amino acids that connect each canonical NADPH sub-region. Ag refers to A. gambiae. Point mutations in Nox2, which occur in variants of X-linked CGD, that correspond to the identified conserved amino acids are indicated. Exceptions to consensus residues are indicated by asterisks and are listed in Additional file 11.