Figure 4

(A) scheme illustrating the location of the sequence conserved regions (SCRs) in RabGDI and REP sequences, as well as the variable size of the region between SCR1A and SCR2. The broken yellow and blue boxes indicate the "gap" in the alignment of these sequences with mammalian REP. Adapted from [13] (B) Neighbor-Joining tree of selected sequences of REP and RabGDI, illustrating that classification of these sequences into one of the two subfamilies is clear. The same tree with bootstrap vaues is provided as supplementary Figure S6. Species codes are Aaeg – Aedes aegypti; Afum – Aspergillus fumigatus; Agam – Anopheles gambiae; Atha – Arabidopsis thaliana; Cbri – Caenorhabditis briggsae; Cele-Caenorhabditis elegans; Cint – Ciona intestinalis; Cmer – Cyanidioschyzon merolae; Cneo – Cryptococcus neoformans; Cpar – Cryptosporodium parvum; Crei-Chlamydomonas reinhardtii; Ddis – Dictyostelium discoideum; Dmel – Drosophila melanogaster; Dpse – Drosophila pseudoobscura; Drer – Danio rerio; Gint-Giardis intestinalis; Hsap – Homo sapiens; Mbre – Monosiga brevicollis; Mmus-Mus musculus; Ngru – Naegleria gruberi; Nvec – Nematostella vectensis; Osat-Oryza sativa; Otau – Ostreococcus tauri; Pber – Plasmodium berghei; Pfal – Plasmodium falciparum; Ppyg-Pongo pygmaeus; Pram – Phytophthora ramorum; Psoj – Phytophthora sojae; Ptri-Populus trichocarpa; Pyoe – Plamodium yoelii; Rnor – Rattus norvegicus; Scer-Saccharomyces cerevisae; Sjap – Schistosoma japonicum; Spom-Schizosaccharomyces pombe; Tbru – Trypanosoma brucei; Tcas – Tribolium castaneum; Tcru – Trypanosoma cruzi; Tpse-Thalassiosira pseudonana; Tnig – Tetraodon nigroviridis; Trub – Takifugu rubripes; Tthe – Tetrahymena thermophila; Xlae – Xenopus laevis; Xtro – Xenopus tropicalis; Ylip – Yarrowia lipolytica (C) Plot of the size of the region between SCR1A and SCR2 in the different species, measured in number of amino acids. The bars are coded according to the colors used in Figure 2 and same color denotes identifiable sequence similarity. The blue and pink dotted perpendicular lines are for reference and indicate the number of amino acids between SCR1B and SCR2 for RabGDI and MRS6, respectively. A red D indicates that the insert contains a predicted disordered region [51], whereas a cross means that such regions are not predicted in the insert.