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Table 3 Search of differences in biologically significant sites between chaetognath ribosomal protein isoforms using Prosite

From: Translational machinery of the chaetognath Spadella cephaloptera: a transcriptomic approach to the analysis of cytosolic ribosomal protein genes and their expression

Protein name

Isoform number

Aa numbers

EST numbers

% identity/overall similarity

Motifs which are different

SSU ribosomal proteins

S7

1

194

30

82.0/97.9

cAMP:1 – PKC:3

 

2

194

1

 

cAMP:2 – PKC:6

S8

1

208

9

is.1/is.2: 96.6/100

amidation:2 – cAMP:2 – myristil:2 – nuclear:3 – PKC:6

 

2

208

8

is.2/is.3: 97.1/100

amidation:1 – cAMP:4 – myristil:2 – nuclear:4 – PKC:8

 

3

208

2

is.1/is.3: 97.1/100

amidation:2 – cAMP:4 – myristil:1 – nuclear:4 – PKC:7

S11

1

156

24

98.1/99.4

nuclear:1

 

2

156

1

 

nuclear:0

S15

1

144

7

99.3/100

N.D.

 

2

144

8

 

N.D.

S16

1

146

9

93.8/96.6

CK2:2

 

2

145

13

 

CK2:3

S17

1

134

19

87.4/96.3

CK2:0 – myristil:0 – nuclear:1 – sulfation:1

 

2

135

10

 

CK2:1 – myristil:1 – nuclear:0 – sulfation:0

S21

1

81

2

83.1/92.8

cAMP:1 – myristyl:2 – PKC:3 – tyr:0

 

2

83

10

 

cAMP:2 – myristyl:1 – PKC:2 – tyr:1

S25

1

115

14

is.1/is.2: 82.8/93.9

cAMP:1 – CK2:0 – myristil:2 – nuclear:1

 

2

114

2

is.2/is.3: 71.7/83.5

cAMP:1 – CK2:1 – myristil:1 – nuclear:1

 

3

127

1

is.1/is.3: 73.2/83.5

cAMP:0 – CK2:0 – myristil:2 – nuclear:0

S28

1

64

8

98.4/100

N.D.

 

2

64

14

 

N.D.

LSU ribosomal proteins

L7

1

245

13

85.7/95.5

cAMP:1 – PKC:2

 

2

243

23

 

cAMP:0 – PKC:3

L15

1

206

1

98.1/99.0

N.D.

 

2

207

1

 

N.D.

L21

1

161

5

97.5/88.1

myristil:2 – PKC:4

 

2

161

2

 

myristil:1 – PKC:5

L22

1

121

10

81.2/92.1

amidation:1 - CK2:2- myristil:4

 

2

128

1

 

amidation:0 - CK2:1- myristil:2

L27

1

136

15

88.2/99.2

myristil:0 – PKC:2

 

2

136

26

 

myristil:1 – PKC:3

L27a

1

145

3

89.6/97.2

CK2:1

 

2

145

10

 

CK2:0

L30

1

114

20

96.5/98.2

PKC:4

 

2

114

6

 

PKC:5

L34

1

130

16

95.4/97.7

cAMP:3 – PKC:1

 

2

131

8

 

cAMP:4 – PKC:2

L35

1

123

13

89.4/98.4

tyr:0

 

2

123

11

 

tyr:1

L36

1

105

16

90.6/98.1

PKC:4

 

2

106

32

 

PKC:2

L37

1

100

9

99.0/99.0

N.D.

 

2

100

8

 

N.D.

L38

1

70

29

92.9/100

asn:1 – CK2:1 – PKC:3

 

2

70

5

 

asn:0 – CK2:0 – PKC:2

L39

1

51

13

is.1/is.2: 96.1/98.0

myristil:0

 

2

51

1

is.1/is.3: 98.0/98.0

myristil:1

 

3

51

1

is.2/is.3: 94.1/96.1

myristil:0

  1. Within a protein family, each of the isoforms are putatively encoded by different types of cDNA, except the L39 isoform-1 and isoform-3 which are encoded by cDNAs belonging to two different subtypes (TTT and TAC respectively) of a same type. Abbreviations: amidation, amidation site; cAMP, cAMP- and cGMP-dependent protein kinase phosphorylation site; CK, casein kinase II phosphorylation site; myristil, N-myristoylation site ; N.D., no difference; nuclear, bipartite nuclear targeting sequence; PKC, protein kinase C phosphorylation site ; tyr, tyrosine sulfation site.