Skip to main content

Advertisement

Figure 10 | BMC Evolutionary Biology

Figure 10

From: Thyroid hormone receptor orthologues from invertebrate species with emphasis on Schistosoma mansoni

Figure 10

DNA binding of SmTRα(Gln182-Ala288) in vitro. Electrphoretic mobility shift assay (EMSAs) shows the DNA binding properties of SmTRα(Gln182-Ala288). DNA binding of SmTRα(Gln182-Ala288) containing 20 aa at the 5' end of the DBD, the DBD and the 40 aa at 3' end of the DBD. Lanes 1, 5, 9, 13, 17, 21, 25 and 29: lysate from the control transcription-translation reaction as negative control. Lanes 2, 6, 10, 14, 18, 22, 26 and 30 contain with lysate with in vitro translated SmTRα (Gln182-Ala288). Lanes 3, 7, 11, 15, 19, 23, 27 and 31 contain lysate with in vitro translated SmTRα (Gln182-Ala288) with a 100 fold of cold specific competitor (unlabeled oligonucleiotides same as the labeled one). Lanes 4, 8, 12, 16, 20, 24, 28 and 32 contain lysate with in vitro translated SmTRα(Gln182-Ala288) with a 100 fold of cold non-specific competitor (5'-CGCGGATCCTGCAGCTCCAG-OH).

Back to article page