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Figure 8 | BMC Evolutionary Biology

Figure 8

From: Thyroid hormone receptor orthologues from invertebrate species with emphasis on Schistosoma mansoni

Figure 8

Protein-protein interaction of SmTRs with SmRXR1. GST pull down shows that both SmTRα and SmTRβ can form a heterodimer with SmRXR1 in vitro. 35S-labeled SmRXR1 was synthesized in vitro using pCITE-SmRXR1 as template and then incubated with GST-SmTRα(LBD), GST-SmTRβ(LBD) or GST (negative control) protein affixed to glutathione-Sepharose beads. The beads were collected, washed and the bound protein was resolved on 10% SDS acrylamide gel and visualized by autoradiography.

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