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Figure 9 | BMC Evolutionary Biology

Figure 9

From: Thyroid hormone receptor orthologues from invertebrate species with emphasis on Schistosoma mansoni

Figure 9

DNA binding properties of SmTRs in vitro. Electrphoretic mobility shift assay (EMSAs) shows the DNA binding properties of SmTRs. A single protein or a combination of two proteins (SmTRα/SmRXR1 or SmTRβ/SmRXR1) were synthesized in a TNT quick coupled transcription/translation system (Promega) and allowed to bind to γ-32P labeled DNA elements containing a half-site, DR0-DR5 and Pal0. A. SmTRα. Lanes 1, 5, 9, 13, 17, 21, 25 and 29 contain lysate from the control transcription-translation reaction as negative controls. Lanes 2, 6, 10, 14, 18, 22, 26 and 30 contain lysate with in vitro translated SmTRα. Lanes 3, 7, 11, 15, 19, 23, 27 and 31 contain lysate with in vitro translated SmTRα and SmRXR1. Lanes 4, 8, 12, 16, 20, 24, 28 and 32 contain lysate with in vitro translated SmRXR1. NS: non-specific binding. B. SmTRβ. Lanes 1, 5, 9, 13, 17, 21, 25 and 29 contain lysate from the control transcription-translation reaction as negative controls. Lanes 2, 6, 10, 14, 18, 22, 26 and 30 contain lysate with in vitro translated SmTRβ. Lanes 3, 7, 11, 15, 19, 23, 27 and 31 contain lysate with in vitro translated SmTRβ and SmRXR1. Lanes 4, 8, 12, 16, 20, 24, 28 and 32 contain lysate with in vitro translated SmRXR1.. Ho: homodimer, Mo: monomer, NS: nonspecific binding.

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