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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Dynamic expression of ancient and novel molluscan shell genes during ecological transitions

Figure 2

RT-PCR analysis of the relative expression levels of 9 genes expressed in shell forming cells and tissues of H. asinina. Histone H1 acted as a control to ensure equal cDNA synthesis efficiency and template loading into the RT-PCR reaction. Coloured rectangles group periods in development that correspond to the occupation of distinct habitats. The yellow rectangle groups planktonic larval stages. The white rectangle indicates animals of a size that occupy CCA dominated habitats. The red rectangle groups juvenile and adult stages that occupy the undersides of coral bommies. Gene abbreviations are as follows: Has-histH1, Haliotis asinina histone H1; Has-ubfm1, Haliotis asinina ubiquitin fold modifier 1; Has-ferrt, Haliotis asinina ferritin; Has-calmbp1, Haliotis asinina calcium binding protein 1; Has-tsfgr1, Haliotis asinina trochophore shell field glycine rich 1; Has-cam1, Haliotis asinina calmodulin 1; Has-vm1, Haliotis asinina veliger mantle 1; Has-vm2, Haliotis asinina veliger mantle 2; Has-lustA, Haliotis asinina lustrin A; Has-som, Haliotis asinina sometsuke. * indicates that RNA was extracted from whole animals rather than biomineralising tissues specifically.

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