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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Dynamic expression of ancient and novel molluscan shell genes during ecological transitions

Figure 3

Sequence and expression analysis of Has-ubfm. (A) Alignment of Has-ubfm to other ubiquitin-like fold modifiers. A conserved glycine residue that is exposed following C-terminal processing and is necessary for conjugation to various target molecules is indicated by *. Sequences are followed by the percentage of sites sharing identity and biochemical similarity. Positions shaded black indicate cases where more than 50% of the residues are identical, and grey where biochemical similarity is shared with the consensus residue. GenBank accession numbers are in brackets. (B) Expression of Has-ubfm is restricted to the expanding edge of the shell field (sf) of trochophore larvae. (C) In pre-torsional veligers expression is associated with the foot (f) primordia. The light refractory operculum is indicated (arrow). The developing mantle (m) lies immediately adjacent to this structure. (D) Expression of Has-ubfm within 5 mm juveniles occurs diffusely throughout the outer fold of the mantle (m). The eye spot (es) is indicated. (E) A magnified view of the boxed region in D reveals Has-Ubfm expression along the anterior edge of the outer fold (of) of the mantle; the ventral-most inner fold is indicated (arrow).

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