Figure 5

Sequence and expression analysis of Has-calmbp1. (A) Has-calmbp1 shares similarity with calcium binding EF proteins from various taxa. EF hand residues identified by Prosite are indicated by *. Sequences are followed by the percentage of sites sharing identity and biochemical similarity. Positions shaded black indicate cases where more than 50% of the residues are identical, and grey where biochemical similarity is shared with the consensus residue. GenBank accession numbers are in brackets. (B) Expression of Has-calmbp1 is restricted to the shell field (sf) of trochophores. (C) Pre-torsional veligers also express Has-calmbp1 in the mantle. (D) Has-calmbp1 is expressed diffusely throughout the mantle of 1 mm juveniles. (E) A magnified view of the boxed region in D reveals Has- calmbp1 transcripts are concentrated along the anterior edge of the outer fold (arrowhead) and the inner mantle fold (arrow) (E).