Figure 7

Sequence and expression analysis of Has-cam1. (A). Has-cam1 shares significant similarity with calmodulin proteins from a range of taxa. Calcium binding EF hand motifs identified by Prosite are indicated by *. Sequences are followed by the percentage of sites sharing identity and biochemical similarity. Positions shaded black indicate cases where more than 50% of the residues are identical, and grey where biochemical similarity is shared with the consensus residue. GenBank accession numbers are in brackets. (B) Has-cam1 transcripts are located in the prototroch of trochophore larva. lateral view. (C) Apical view of trochophore larva. (D) Pre-torsional veligers also express Has-cam1 in the prototroch. The foot (f) and mantle (m) primordia are indicated. (E) Competent veligers express Has-cam1 in the mantle (arrow). The operculum (op) and eye spot (es) are indicated. (F) 1 mm juveniles express Has-cam1 within the gills (g) and the mantle (arrows). (G) Magnified view of the boxed region in F. Has-cam1 transcripts are restricted to the anterior edge of the outer mantle fold (arrowhead). The inner mantle fold is also visible (arrow).