Table 1 Sequences of primers used for RACE and RT-PCR analysis

GeneRacer™ 5'

5'-CGACTGGAGCACGAGGACACTGA-3'

64°C

Reverse GSP

5'-CAGACCATCTCGGTGGGGGAGTA-3'

GeneRacer™ 5' Nested

5'-GGACACTGACATGGACTGAAGGAGTA-3'

55°C

Reverse Nested GSP

5'-CACAGTGCACACGAAGAAGATAGTG-3'

GeneRacer™ 3'

5'GCTGTCAACGATACGCTACGTAACG-3'

55°C

Forward GSP

5'-ACTCCACTGGCAGCCACAGAGG-3'

GeneRacer™ 3' Nested

5'CGCTACGTAACGGCATGACAGTG-3'

56°C

Forward Nested GSP bovine

5'-CCCTTCCTGTGGCCTCTGATACTC-3'

Forward Nested GSP pig

5'-ACCAGCACCCACGGAGGCACAGACC-3'

Forward Nested GSP rat

5'-CCCTGCCAGGGAGTTCAGATCTC-3'

Forward hPSGL-1/AflII

5'-AGCCTTAAG CCACCATGCCTCTGCAACTCC-3'

54°C

Forward b, pPSGL-1/AflII

5'-TATCTTAAG CCACCATGTTTCTGCAACTCC-3'

Forward rPSGL-1/AflII

5'-CGCCTTAAG CCACCATGTTCCCACACT-3'

Forward ePSGL-1/AflII

5'-AGCCTTAAG CCACCATGCCTCTGCCGCTC-3'

Reverse h, b, p, r, ePSGL-1/AgeI/ClaI

5'-TGGACCGGT ATCGAT AGGGAGGAAGCTGTG-3'

Forward h, b, p, rFucT-VII

5'-TCCTTGTCTGGCACTGG-3'

50°C

Reverse h, b, p, rFucT-VII

5'-GCGGTGCTGGGAGTTCT-3'

Forward h, b, p, rβ-actin

5'-GAGACCTTCAACACCCC-3'

50°C

Reverse h, b, p, rβ-actin

5'-GTGGTGGTGAAGCTGTAGCC-3'