Skip to main content
Figure 4 | BMC Evolutionary Biology

Figure 4

From: Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer

Figure 4

Activation of Hdm2 and p21 promoters by human and axolotl p53 in H1299 cells. (A-C) Dual-luciferase assays in H1299 cells with Hdm2 promoter at 37°C, 30°C and 25°C. (D-F) Dual-luciferase assays in H1299 cells with the human p21 promoter at 37°C, 30°C and 25°C. Luciferase activities stimulated by human or axolotl p53 was significantly different than non-p53 controls (at least p < 0.05, data not shown). Error bars are ± s.e.m. human and axolotl p53 luciferase transactivation were significantly different during the same assay using the Hdm2 promoter (A-C) or the human p21 promoter at 37°C (D) (at least p < 0.01). Each assay was performed in triplicate at least 3 separate times. (G) Inhibition of the activation of Hdm2 promoter by the combined expression of human and axolotl p53 in H1299 cells. All luciferase activities were significantly different than non-p53 controls (at least p < 0.05, data not shown). Error bars are ± s.e.m. human + axolotl and human p53 luciferase transactivation were significantly different during the same assay using the Hdm2 promoter at 37°C (p = 0.006). Each assay was performed in triplicate. (H) Growth assays at 37°C on H1299 cells transfected with human or axolotl p53 protein.

Back to article page