Figure 5
From: Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer
Activation of endogenous Hdm2 in H1299 cells by the axolotl p53 protein. Expression of the human p53 target gene (Hdm2) 24 h post transfection in H1299 cells mock transfected and transfected with the axolotl p53 without and with pifithrin-α grown at 30°C. RT-PCRs were also performed with the housekeeping gene glyceraldehyde phosphate dehydrogenase (Gapdh) to control for the amount of total RNA.