Figure 7
From: Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer
Detection of p53 protein in AL1 cells. (A & C) Western blot analysis of phospho-ser15 p53 in AL1 cells exposed to UV or treated with MNNG. (B) RT-PCR analysis of p53 target gene, Gadd45 (a p53 target gene cloned in axolotl [57, 58]), in control treated axolotl AL1 cells, cells exposed to UV (6h post-irradiation) and cells exposed to UV & treated with pifithrin-α. Both Gapdh and Ef1α were used as controls to demonstrate that the effects of UV and UV plus pifithrin-α were specific for Gadd45. (D-E) Western blot analysis of total p53 protein (CM5 antibody) on AL1 cells exposed to UV or treated with MNNG.