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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Functional evolution of the vitamin D and pregnane X receptors

Figure 3

Enzyme induction in cultured primary zebrafish hepatocytes. (A) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and 3-methylcholanthrene (3-MC) both increase EROD activity in zebrafish hepatocytes, relative to vehicle-only control, following a 48-hour exposure. In contrast, 3,3'-diindoylmethane (DIM) causes a reduction in EROD activity relative to vehicle-only control. (B) 5α-androstan-3α-ol (androstanol), 5α-cyprinol 27-sulfate, phenobarbital, and TCDD cause a concentration-dependent increase in testosterone 6β-hydroxylation activity in primary zebrafish hepatocytes, relative to vehicle control, following a 48-hour exposure. (C) Androstanol, 5α-cyprinol 27-sulfate, phenobarbital, and TCDD all activate recombinant zebrafish PXR using a luciferase-based reporter assay system in HepG2 liver cells. All values are normalized relative to 20 μM androstanol (which is assigned an efficacy of 1) and to β-galactosidase expression. (D) Phenobarbital (PB; 2 and 3 mM), TCDD (1 nM), and androstanol (0.2 mM) all increase flurbiprofen 4-hydroxylation activity in primary zebrafish hepatocytes, relative to vehicle control, following a 48-hour exposure.

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