Cryptic diversity and deep divergence in an upper Amazonian leaflitter frog, Eleutherodactylus ockendeni

Table 3 Microsatellite allele sizes

Locus	Lowland	Upland	Comment
Eloc-Laurel&Hardy	166–262	---	Generally non-amplifying in Upland and twice the repeat length vs. Lowland
Eloc-Bert&Ernie	106–194	202–250	Non-overlapping allele sizes
Eloc-Thelma&Louise	151–247	243–443	Non-overlapping allele sizes except in two individuals
Eloc-Batman&Robin	208–282	300–404	Non-overlapping allele sizes (excluding four Lowland individuals with possible large alleles sizes, 376–464)
Eloc-Beauty&Beast	145–221	185–241	Unequal allele size ranges

Microsatellite profiles (in total fragment size) by locus examined in E. ockendeni from the Napo River area (localities 3 – 11). Microsatellite allele sizes (total fragment bp) were larger in the Upland than Lowland Clade. Using E. ockendeni primers [105], one locus exclusively amplified in the Lowland Clade, suggesting it is non-functional in the Upland Clade.

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