Parsimony tree for Cucumis based on sequences from the nuclear internal transcribed spacer, rooted on Muellerargia as in Fig. 1. Bootstrap values (> 65%) at branches are based on 1000 replicates. The genera marked with red lines are nested in Cucumis, and their species will need to be transferred to make Cucumis monophyletic. Species with the letters GM (Garcia-Mas) are from , while species labeled HS were generated for this study. The GenBank sequence labeled '?Oreosyce africana?' is from misidentified material (see text).