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Table 3 Background information of the sampled populations and summary of the molecular evolutionary analysis in DnaSP.

From: Recombinational micro-evolution of functionally different metallothionein promoter alleles from Orchesella cincta

  

Plombières

Roggebotzand

[Cd]tot soil (mg/kg) (Janssens, unpublished)

30.45 ± 14.82

0.17 ± 0.05

Allele frequencies per population (%) (Janssens, unpublished)

pmt A1

32.9

47.0

 

pmt A2

7.6

15.7

 

pmt B

24.7

10.4

 

pmt C

11.4

2.2

 

pmt D1

10.1

13.4

 

pmt D2

8.9

1.5

 

pmt E

0.6

6.0

 

pmt F

1.9

2.9

 

pmt BAL

1.9

0.7

 

N

79

67

Sample size (number of alleles)

158 (9)

134 (9)

S (ç)

201(214)

201 (214)

Hd

0.798 ± 0.017

0.726 ± 0.032

Ï€

0.02634 ± 0.00159

0.01491 ± 0.00162

Tajima's D

0.17187 NS

-1.3781, NS

Fu and Li's D

2.88149, p < 0.02**

-0.75998, NS

Fu and Li's D*

2.77359, p < 0.02 **

-2.13596, NS

Average constitutive mt expression. MNE relative to β-actin (Roelofs, unpublished)

0.52 ± 0.18

0.04 ± 0.00

Induced (1 μmole Cd/g food) mt expression. MNE relative to β-actin (Roelofs, unpublished)

2.63 ± 0.57

1.18 ± 0.66

Mean Cd excretion efficiency per moult (Posthuma 1993)

45%

38%

  1. Total cadmium (Cd) content of the soil; allele frequencies of the pmt alleles; S, total number of variable (segregating) sites; η, total number of mutations; Hd, haplotype diversity ± SD; π, nucleotide diversity per site ± SD; Tajima's D, Fu and Li's D and Fu and Li's D*, mean normalized expression, p-value of the G-test performed on the reconstituted dataset (df = 8), MNE, mean normalized expression at mRNA level; Cd excretion efficiency; percentage of Cd-induced growth reduction.