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Figure 2 | BMC Evolutionary Biology

Figure 2

From: RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation

Figure 2

Species-specific alternative splicing patterns. (A) Analysis of species-specific alternative splicing patterns using an RT-PCR-based strategy. 1: D. melanogaster; 2: A. mellifera; 3:B. mori; 4: T. castaneum. Primers used for amplification of splice products were DmDa-5-1 and DmDa-3-1 for D. melanogaster, AmDa-5-3 and AmDa-3-1 for A. mellifera, BmDa-5-4 and BmDa-3-1 for B. mori, TcDa-5-1 and TcDa-3-1 for T. castaneum, respectively (Table 1). The migration positions of PCR products corresponding to transcripts with one or two alternative duplicated exon variants are indicated. Because the sequence between the specific primers in T. castaneum is smaller than in other species, its band is smaller. (B) Comparison of the boundary sequences of sites in exon 2 and alternative exons 3 among nAChR alpha6 orthologs of D. melanogaster (Dme), B. mori (Bmo), T. castaneum (Tca) and A. mellifera (Ame). Direct sequencing of these RT-PCR products (A) confirmed that these duplicated exons are alternatively spliced. Different nucleotides in the alternative exons 3a and 3b (in box) showed a mixed signal.

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