Skip to main content

Table 2 Substitutions observed among guppy LWS opsins, and comparable mutants made in bovine RH1 and human LWS.

From: Long-wavelength sensitive visual pigments of the guppy (Poecilia reticulata): six opsins expressed in a single individual

Observed Substitutions Among Guppy Opsins1

Comparable Mutants Made in Bovine RH12 [48]

Transducin Activity Relative to Wildtype RH1 [48]

S256C

S256A

60%

K261N

K261L

98%

R264K

K264L

72%

H247R, A248D

K247T, E248Q

87%

K261N, R264K

K261L, K264L

61%

E263Q, R264K

E263Q, K264L

110%

Observed Substitutions Among Guppy Opsins1

Comparable Mutants Made in Human LWS2 [45, 46]

Effect on LWS Spectral Tuning LWS [45, 46]

I230T

I230T

2–3 nm

A233G

A233S

2–3 nm

Y277F

Y277F

6–10 nm

T285A

T285A

10–16 nm

Y277F, T285A

Y277F, T285A

18–27 nm

  1. 1 Substitutions observed among guppy LWS opsins; refer to text and Fig. 4 for more details
  2. 2 Mutants created in bovine RH1 and human LWS by site-directed mutagenesis